Validation of Quantification Process for Total Polyphenol and Flavonoid in Propolis Hydrogel by UV Spectrophotometry

Abstract

Propolis is a resin rich in polyphenols and flavonoids, providing strong antibacterial, antioxidant, and wound-healing properties. Therefore, propolis hydrogels are therefore promising wound dressing materials that accelerate the healing of minor injuries. This study aimed to validate a Ultraviolet spectrophotometric method for the quantification of total polyphenol and total flavonoid content in propolis hydrogels. The quantifications were validated for their selectivity, linearity, accuracy and precision. The results demonstrated excellent linearity for polyphenols, and flavonoids over a concentration range of (10-120) µg/mL at 754 nm and 427 nm, respectively. Furthermore, the method exhibited high specificity, accuracy (recovery rates between 95% and 102%), and precision (relative standard deviation < 2%) for both analytes. The TPC and TFC in the propolis hydrogel were (4.2392 ± 0.0495) mgGAE/g and (2.1733 ± 0.0817) mgQE/g respectively. These validation results are crucial for establishing robust quality control criteria in the manufacturing of propolis hydrogels.