Recombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli

Abstract

MGG06069 is a polysaccharide monooxygenase protein that originated from the fungus
that causes rice blast, Magnaporthe oryzae. It has the potential to speed up the
cellulolysis process, which can be beneficial for industry. In this study, the full-length
MGG06069 protein and its polysaccharide monooxygenase domain were expressed in
Escherichia coli in order to produce recombinant proteins on a large scale for further
enzymatic experiment. It was shown that the amount of the recombinant polysaccharide
monooxygenase domain protein was much higher compared to that of the full-length
protein. In addition, only one band of expected size was obtained for the recombinant
PMO domain protein. These results suggest the purified polysaccharide
monooxygenase domain of MGG06069 that can be used for enzymatic assays
in the future.
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